CRISPR Screening Technician

CRISPR Screening Technician Arinale Biosciences Incorporated

Arinale Biosciences is a biotechnology startup operating in stealth mode after recently being spun out of one of New York City’s premier academic medical centers. Occupying a unique position at the nexus of scientific discovery and healthcare, Arinale’s platform for rapid development of paradigm-changing therapeutics will extend the benefits of precision medicine far beyond current limitations and fundamentally improve human. We are helping create a future where every diagnosis is clearer, every decision is more confident, and every patient has more hope. Mission Guided by vision and powered by innovation, we will catalyze the discovery and development of a new generation of transformative precision therapies. Role We are seeking a mid-to-senior level laboratory technician specializing in CRISPR screening to independently plan and execute pooled CRISPR loss- and gain-of-function screens in mammalian cell lines. A successful candidate will own the full experimental workflow end to end, including sgRNA library design and construction, high-titer lentiviral production, low-MOI transduction, antibiotic selection, FACS-based cell sorting, cell culture, genomic DNA isolation, and sgRNA library preparation for next-generation sequencing. Ideal candidates are meticulous, safety-conscious bench scientists who thrive on troubleshooting long, multi-stage workflows and take full ownership of screen quality from library design through sequencing handoff.

Location This position is in New York (NY) and requires full-time, in-person work in a laboratory setting.

Responsibilities Design, construct, and validate sgRNA expression libraries for pooled and/or arrayed screens, including guide selection, development of cloning strategies, and plasmid library QC Produce, concentrate, and titer high-quality lentivirus preparations at scale to support genome-wide or focused sub-pool screens Execute low-MOI transductions and antibiotic selection while preserving full library representation Perform FACS-based sorting for phenotypic screen readouts, including gating strategy design and post-sort recovery Maintain mammalian cell culture across all stages of a screen, from pilot to full scale Isolate high-quality genomic DNA and prepare barcoded sgRNA libraries for next-generation sequencing Track coverage, representation, and QC metrics at every stage and proactively flag deviations Author and maintain detailed protocols, records, and SOPs to ensure reproducibility across screens Coordinate with sequencing team, vendors, and bioinformatics collaborators on sample handoff and specifications Requirements B.S. in Molecular Biology, Cell Biology, Biochemistry, Genetics, or a related field (M.S. a plus) 3+ years (mid-level) or 6+ years (senior) of full-time, hands-on mammalian cell culture and molecular biology experience 1+ years (mid-level) or 3+ years (senior) of direct, hands-on experience with lentiviral production, titering, and transduction 1+ years (mid-level) or 3+ years (senior) of experience executing all components of a pooled CRISPR screen (plasmid library construction, virus production, selection, and sequencing library prep) 1+ years (mid-level) or 3+ years (senior) of experience preparing samples for flow cytometry/FACS, plus a working understanding of sorting and gating principles 1+ years of proficiency in genomic DNA extraction, PCR, and NGS library preparation techniques Meticulous attention to detail and rigorous documentation habits Strong troubleshooting skills across long, multi-step, interdependent workflows Ability to work independently and manage multi-week experimental timelines Standout Experiences Independent ownership of a full pooled CRISPR screen from library design through sequencing handoff Experience with genome-wide screening libraries (e.g., Brunello, GeCKO, Cellecta) Familiarity with screen-analysis pipelines (e.g., MAGeCK, BAGEL, drugZ) and ability to interpret QC and hit-calling outputs Hands-on operation of a cell sorter (e.g., BD FACSAria-class instrument) Experience with CRISPR screen variants beyond knockout (CRISPRi, CRISPRa, base editing) Publication or major technical contribution to a CRISPR screening study Basic scripting ability (Python or R) to support screen QC and data handoff Compensation and Benefits

Mid-level: $75,000-85,000 base salary + bonus Senior-level: $90,000-$100,000 base salary + bonus Stock options Health/Dental/Vision coverage